Structural Biology of Bacterial Macromolecular Complexes

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Terradot Lab

 


Bacterial infections rely on the micro-organisms’ ability to adapt to its environment, subvert the immune system and multiply into its host. Our laboratory uses Biochemistry, X-ray Crystallography, Cryo-electron microscopy  and Biophysics techniques to investigate how bacteria infect human tissues. We focus our investigations on a particularly important one, Helicobacter pylori, associated with various human diseases, including gastric ulcers and stomach cancers. Our goal is to provide the molecular details of key infection steps to drive new antibiotics discovery. We use Structural Biology, a discipline that allows for a near atomic view of macromolecules and how they accomplish their functions.



Institut de Biologie et

Chimie des Protéines


UMR5086 - Molecular Microbiology

and Structural Biochemistry


7, passage du Vercors

Lyon 69367 Cedex, France




Contact: @LaurentTerradot


Phone:+33 (0)472 72 2652

Fax: +33 (0)472 72 2604

laurent.terradot(a)ibcp.fr

 


If you are interested by our projects, do no hesitate to contact me.

We are always looking for enthusiastic students and scientists to join us !

Selected publications (complete list here)



  2. - Liu L, Gueguen-Chaignon V, Gonçalves IR, Rascle C, Rigault M, Dellagi A, Loisel E, Poussereau N, Rodrigue A, Terradot L•, Condemine G•. (2019) A secreted metal-binding protein protects necrotrophic phytopathogens from reactive oxygen species. Nature Communications 10(1):4853. doi: 10.1038/s41467-019-12826-x.

  3. - Rigard M, Bröms JE, Mosnier A, Hologne M, Martin A, Lindgren L, Punginelli C, Lays C, Walker O, Charbit A, Telouk P, Conlan W, Terradot L ●, Sjöstedt A ●, Henry T ●. (2016) Francisella tularensis IglG Belongs to a Novel Family of PAAR-Like T6SS Proteins and Harbors a Unique N-terminal Extension Required for Virulence. PLoS Pathogens 12(9):e1005821. doi: 10.1371/journal.ppat.1005821.

  4. - Felicori, L, Jameson, KH, Fogg, MJ, Roblin, P, Garcia, T, Ventroux, M, Cherrier, MV, Bazin, A, Noirot, P, Wilkinson, AJ, Molina F ●, Terradot L ●, Noirot-Gros MF ● . (2016) Structural characterization of the replication initiation controller YabA reveals interdomain flexibility. Nucleic Acid Research. 44(1):449-63.

  5. - Flayhan, A., Bergé, C., Baïlo, N., Doublet, P., Bayliss, R. and Terradot, L.●. (2015) The structure of Legionella pneumophila LegK4 type four secretion system (T4SS) effector reveals a novel dimeric eukaryotic-like kinase. Scientific Reports 5:14602. doi: 10.1038/srep14602.

  6. - Bazin, A., Cherrier, M.V., Gutsche, I., Timmins, J., Terradot, L.●. (2015) Structure and primase-mediated activation of a bacterial dodecameric replicative helicase. Nucleic Acid Research 43(17):8564-76.

  7. - Kaplan-Turkoz, B., Jimenez-Soto, L.F., Dian, C., Ertl, C., Remaut, H., Louche, A., Tosi, T., Haas, R.●, Terradot, L.● (2012) Structural insights into Helicobacter pylori oncoprotein CagA interaction with beta1 integrin. PNAS 109: 14640-14645.

  8. - Stelter, M., Gutsche, I., Kapp, U., Bazin, A., Bajic, G., Goret, G., Jamin, M., Timmins, J., Terradot, L.● (2012) Architecture of a dodecameric bacterial replicative helicase. Structure 20: 554-564.

  9. - Dian, C., Vitale, S., Leonard, G.A., Bahlawane, C., Fauquant, C., Leduc, D., Muller, C., de Reuse, H., Michaud-Soret, I. ●, Terradot, L. ● (2011) The structure of the Helicobacter pylori ferric uptake regulator Fur reveals three functional metal binding sites. Mol. Microbiology 79(5),1260-75.

  10. - Natrajan, G., Noirot-Gros, M.F., Zawilak-Pawilk, A., Kapp, U., and Terradot, L●. (2009) Structure of a DnaA/HobA complex from Helicobacter pylori: insights into regulation of DNA replication in Gram-negative bacteria. PNAS. 106, 21115-20.